Determination of constituents of aurantio-obtusin, emodin, rhubarb, and physcion in Cassiae Semen by multi-components by single mark / 中草药

Objective: To establish a quantitative analysis of multi-components by single mark (QMSA) for determining the contents of four anthraquinones (aurantio-obtusin, emodin, rhubarb, and physcion) from Cassiae Semen, and verify the feasibility of this method in quality control. Methods: The medicinal material of Cassiae Semen was used as the research object, emodin was used as reference, two kinds of correction methods were used to establish the relative correction factor (fk/s) of aurantio-obtusin, emodin, and physcion. The amount of each component was calculated, at the same time using the external standard method for determination of the three components, comparing the difference between calculated values of QMSA and measured value to validate the feasibility and accuracy of QMSA. Results: The relative correction factors of aurantio-obtusin, emodin, and physcion were established, without significant difference between the calculated values by QMSA and measured values. Conclusion: The experimental QMSA method can accurately determine the contents of the four anthraquinones in Cassiae Semen, and can be used in the multi-index evaluation in Cassiae Semen anthraquinones constituents.

Study of reason of "the older, the better" of Pericarpium Citri Reticulatae (PCR) which contributed increase of flavonoids / 中国中药杂志

This study is to explore the reason of "the older, the better" of PCR and itsincrease of flavonoids. We identified the fun- gus isolated from the PCR using microscopic and molecular identification. HPLC method was used to determine the content of 4 fla- vonoids and to clarifythe regularity of them; UV spectrophotometry method was used to determine the total content of flavonoids; reverse thinking was applied to screen the fungus that have close relation to the change of flavonoids. Finally, we have isolated and identified 25 fungusfrom the PCR, which belong to 2 genus and 4 species, including pencillium commune, P. minioluteeum, P. citrinum, Aspergillus flavus and A. niger. The content of flavonoids was increased in the mildew PCR due to A. niger and other fungus. Therefore, "the ol- der, the better" of PCR had its scientific reason that the increase of flavonoids had a close relation of the metabolic activity of A. niger and other fungus.